Protein G Agarose Resin 4 Rapid Run™ - 5 mL
High Pressure:
Protein G Rapid Run™ Agarose Beads
BULK RESINS
Protocol:
- The Binding capacity of Protein G RAPID RUNTM Agarose Resin depends on the source of the immunoglobulin.
- There might be deviations in binding capacities for different immunoglobulins derived from the same species, even if they are of the same subclass.
- Maxanim Recombinant Protein G contains only IgG binding domains.
- The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG Binding capacity.
- It is produced in E. coli, and has a predicted molecular mass of approximately 21.6 kDa, but it migrates with an apparent molecular mass of 32 kDa in SDS-PAGE.
- The pI of Protein G is 4.1 and the pH stability 2 – 10. Optimal binding occurs at pH 5.0, although binding is also good at pH 7.0 – 7.2.
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