Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP)

Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) encodes Firefly Luciferase (FLuc), a widely used bioluminescent reporter protein for gene expression analysis, live-cell imaging, and high-throughput screening assays. This mRNA is optimized with an Anti-Reverse Cap Analog (ARCA) for efficient translation initiation, while 5-methylcytidine triphosphate (5mCTP) and pseudouridine triphosphate (ψUTP) modifications enhance mRNA stability, reduce innate immune activation, and increase protein expression in mammalian cells. Firefly luciferase emits bright bioluminescence upon substrate addition, making it ideal for sensitive and quantitative real-time monitoring of cellular processes.