EZ Cap™ Firefly Luciferase mRNA (5-moUTP)
EZ Cap™ Firefly Luciferase mRNA (5-moUTP) encodes Firefly Luciferase (FLuc), a widely used bioluminescent reporter for gene expression analysis, live-cell imaging, and high-throughput screening assays. This mRNA is synthesized with an optimized Cap1 structure, ensuring efficient translation and stability in mammalian cells. The 5-methoxyuridine triphosphate (5-moUTP) modification enhances mRNA stability, reduces immune activation, and prolongs expression duration. Upon substrate addition, Firefly Luciferase produces bright and quantitative bioluminescence, making it an ideal tool for sensitive real-time molecular tracking and cellular analysis.