EZ Cap™ Cas9 mRNA (m1Ψ)

EZ Cap™ Cas9 mRNA (m1Ψ) encodes Streptococcus pyogenes Cas9 (SpCas9), a widely used endonuclease for CRISPR-based genome editing applications. This mRNA is synthesized with an optimized Cap1 structure, ensuring efficient translation and prolonged stability in mammalian systems. The incorporation of N1-Methylpseudouridine (m1Ψ) modification enhances mRNA stability, reduces innate immune activation, and increases protein expression efficiency. When combined with a guide RNA (gRNA), EZ Cap™ Cas9 mRNA (m1Ψ) enables precise gene knockout, insertion, and correction, making it ideal for genome engineering, functional genomics, and therapeutic research.